The Athena pipeline is a proprietary variant calling pipeline specialized in analyzing Next Generation Sequencing (NGS) viral data generated by an Illumina sequencing platform. The need for successful development of SARS-CoV-2 antiviral compounds and vaccines is at its peak resulting in a necessity for biological interpretation of viral genomics and the underlying mechanisms. Reliably detecting genomic events in NGS data is of utmost importance for data interpretation and a better understanding of the virus. The Athena bioinformatics pipeline contributes specifically to this field to aid research teams in their investigations. In this webinar, the use of Athena on SARS-CoV-2 datasets will be demonstrated on the Seven Bridges Platform.